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. 2018 Dec 11;9:612. doi: 10.3389/fgene.2018.00612

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Copyright © 2018 Sollazzo, Genchi, Paglia, Di Giacomo, Pession, de Biase and Grifoni.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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MYC and PI3K^CAAX overexpression increases mitotic activity. (A,A1) Immunostaining for PH3 (red) on wing discs from late yw; hh-Gal4, UAS-GFP/UAS-dm larvae. (B) Graph comparing the PH3-positive nuclei counted in the P (green bar) and A (grey bar) compartments, ^∗∗∗p ≤ 0.001. (C,C1) Immunostaining for PH3 (red) on wing discs from late yw/yw, UAS-PI3K^CAAX; hh-Gal4, UAS-GFP/+ larvae. (D) Graph comparing the PH3-positive nuclei counted in the P (green bar) and A (grey bar) compartments, ^∗∗p ≤ 0.01. (E) Graph comparing the PH3-positive nuclei counted in the P compartments of yw; hh-Gal4, UAS-GFP/UAS-dm (striped green bar) and yw/yw, UAS-PI3K^CAAX; hh-Gal4, UAS-GFP/+ (dotted green bar) larvae, ^∗∗∗p ≤ 0.001. Basic genotypes are indicated on the left of the figure panels and under the graphs. P compartments are outlined in A1,C1, and disc axes are indicated in A,C. Magnification is 400×.