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. 2018 Dec 11;9:612. doi: 10.3389/fgene.2018.00612

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Copyright © 2018 Sollazzo, Genchi, Paglia, Di Giacomo, Pession, de Biase and Grifoni.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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About one/third of lgl^- clones overgrows at the expense of the surrounding wild-type tissue in a MYC-overexpressing background. (A,A1) Immunostaining for Lgl (red) and MYC (cyan) on wing discs from late w; l(2)gl⁴, FRT40A/Ubi-GFPnls, FRT40A; hh-Gal4/UAS-dm larvae. The arrow points to a posterior wild-type twin clone and the arrowhead indicates a scattered lgl^-/- clone with no obvious wild-type twin in the posterior hinge region (A1). (B,C) Graphs comparing the average clone area of lgl^-/- (black triangles, black in the images) and wild-type twins (grey triangles, double red in the images) in the P (B) and A (C) compartments, ^∗∗∗p ≤ 0.001. The basic genotype is indicated on the left of the figure. The P compartment is outlined in A1, and disc axes are indicated in A. Magnification is 400×.