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. 2018 Dec 11;9:1828. doi: 10.3389/fpls.2018.01828

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Copyright © 2018 Sgamma, Masiero, Mali, Mahat and Slater.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Aristolochia subgenus Siphisia-specific quantitative real-time (A) Ct values. The lighter bars indicate primer dimers or non-specific products as per melting curve data. qPCR was performed using three biological replicates with three technical replicates for each sample. Error bars represent Standard deviation. (B) Melting curve for all samples run with Siphisia-specific primers. (C) Agarose gel electrophoresis of PCRs using Siphisia-specific primers. The white arrows point to the products with the expected size (88 bp). Gel lanes: (1) HyperLadder^TM 25 bp (Bioline); (2) Positive control; (3) Negative (no template) control; (4) A. californica; (5) A. kaempferi; (6) A. baetica; (7) A. clematitis; (8) S. tetrandra; (9) S. glandulifera; (10) S. rotunda; (11) C. trilobus; (12) C. laurifolius; (13) S. acutum; (14) A. europaeum; (15) A. arifolium; (16) A. fudsinoi; (17) S. alpine; (18) D. glaucescens; (19) M. dahuricum; (20) S. quercifolia.