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Detection and quantitation of Aristolochia subgenus Siphisia DNA using the Siphisia-specific primers. ΔCt values were calculated as the difference between the mean Ct value of the Siphisia-specific amplification and the mean Ct value of the internal control 5.8S amplification. Aristolochia S-5 gBlock was used as calibrator sample. qPCR was performed using three biological replicates with three technical replicates for each sample. Error bars represent Standard deviation.
