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. 2018 Dec 11;9:1828. doi: 10.3389/fpls.2018.01828

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Copyright © 2018 Sgamma, Masiero, Mali, Mahat and Slater.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Quantitation of Aristolochia subgenus Siphisia DNA in admixtures. ΔCt values were calculated as the difference between the mean Ct value of the target Siphisia-specific amplification and the mean Ct value of the internal control 5.8S amplification. Aristolochia S^-5 gBlock neat and dilutions 1:10, 1:100, and 1:1000 were used as calibrator samples for the corresponsive dilution mix. qPCR was performed using three biological replicates with three technical replicates for each sample. Error bars represent Standard deviation.