Table 3.

Aristolochia-specific and generic primers and annealing temperature (Ta) used in quantitative real-time PCR.

Primer name	Sequence	Ta	Expected size (bp)
Aristolochia-ITS2 F	5′- CTCGATCGGAGGGTGCGTG -3′	62°C	88
Aristolochia-ITS2 R	5′- GCCAAGGCTTTCAGCCAACC-3′
Generic 5.8 F	5′- GACTCTCGGCAACGGATA-3′	60°C	93
Generic 5.8 R	5′- GATGGTTCACGGGATTCTG-3′