Fig. 6.
qRT-PCR for RAN gene expression after 24 and 48 h transfection of PLGA-shRNA-1 NP and PLGA-shRNA-4, where cells were transfected with 2.0 μg ml−1 shRNA-1 and 2.0 μg ml−1 shRNA-4. Control was blank PLGA-shRNA NP. Relative gene expression was measured using β-actin as a house keeping gene