Table I.
Physical characterisation of shRNA-loaded NP, where average size, zeta potential, polydispersity index (PDI), encapsulation efficiency (EE) and encapsulated drug amount were determined*
| Copolymers |
size
(nm) |
PDI |
zeta potential
(mV) |
EE
(%) |
Drug loading
(μg per mg NP) |
|---|---|---|---|---|---|
| PLGA-shRNA-1 | 426.41 ± 26.23 | 0.414 ± 0.144 | −0.332 ± 0.66 | 74.94 ± 1.32 | 0.10 ± 0.01 |
| PLGA-PEG-5%-shRNA-1 | 251.61 ± 14.71 | 0.231 ± 0.015 | −0.224 ± 1.03 | 75.10 ± 7.67 | 0.10 ± 0.10 |
| PLGA-PEG-10%-shRNA-1 | 272.36 ± 9.82 | 0.336 ± 0.018 | −0.092 ± 0.37 | 66.31 ± 3.74 | 0.09 ± 0.05 |
| PLGA-shRNA-4 | 408.45 ± 20.18 | 0.348 ± 0.012 | −2.71 ± 0.039 | 85.18 ± 2.52 | 0.11 ± 0.03 |
| PLGA-PEG-5%-shRNA-4 | 289.93 ± 13.24 | 0.201 ± 0.020 | −1.81 ± 0.023 | 84.02 ± 5.81 | 0.11 ± 0.08 |
| PLGA-PEG-10%-shRNA-4 | 237.71 ± 23.36 | 0.265 ± 0.011 | −1.17 ± 0.044 | 80.01 ± 1.06 | 0.11 ± 0.02 |
*Data are mean ± SD with n = 6