Fig. 6.

C/3′NCR-mir(T) virus is immunogenic to the rhesus macaques. a Experimental design of the study. b Mean viremia ± SD (shown as upper error bars) post immunization with ZIKV-NS3m or C/3′NCR-mir(T) viruses. c Neutralizing antibody response in immunized monkeys before and after the challenge with 10⁵ pfu of wt ZIKV. Horizontal lines denote geometric mean ± geometric SD (shown as error bars). d Mean viremia + SD (shown as upper error bars) after the challenge with wt ZIKV. Differences between viral titer and between ZIKV-specific NA titer in serum were compared using two-way ANOVA and one-way ANOVA, respectively (**p < 0.01, ***p < 0.001; ns denotes not statistically significant (p > 0.05). The dashed lines indicate the limit of virus detection (0.7 log₁₀(pfu/mL)) for serum b and d, or the limit of NA titer detection c