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. 2018 Dec 17;8:17892. doi: 10.1038/s41598-018-35586-y

Figure 4.

Figure 4

Inhibition of E. coli YgbP by MEPN3. (a) Steady state kinetics at variable MEP concentrations and fixed MEPN3 concentrations (0 µM, r2 = 0.99; 20 µM, r2 = 0.98; 40 µM, r2 = 0.99; 60 µM, r2 = 0.99). (b) Double reciprocal plot of (a) (0 µM, r2 = 0.99; 20 µM, r2 = 0.99; 40 µM, r2 = 0.98; 60 µM, r2 = 0.99) (c) Replot of slope and intercept of (b) (slope, r2 = 0.98; intercept, r2 = 0.95). (d) Steady state kinetics at variable CTP concentrations and fixed MEPN3 concentrations (0 µM, r2 = 0.98; 30 µM, r2 = 0.99; 60 µM, r2 = 0.99; 90 µM, r2 = 0.98). (e) Double reciprocal plot of (d) (0 µM, r2 = 0.99; 30 µM, r2 = 0.98; 60 µM, r2 = 0.98; 90 µM, r2 = 0.99). (f) Replot of slope and intercept of (e) (slope, r2 = 0.99; intercept, r2 = 0.95). Mean and SEM values are displayed, n ≥ 3.