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. 2018 Jun 8;99(5):1010–1021. doi: 10.1093/biolre/ioy132

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© The Author(s) 2018. Published by Oxford University Press on behalf of Society for the Study of Reproduction.

This article is published and distributed under the terms of the Oxford University Press, Standard Journals Publication Model (https://academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model)

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Figure 3. — Hyperactivation at different A23187 concentrations measured by CASA. Sperm treated with different A23187 concentrations (0.5, 1, 5, 10 μM) were monitored at different time points 1, 2, 3, 10, 60 minutes to evaluate hyperactivation. (A) Control spermatozoa (without A23187) during capacitation. Spermatozoa treated with A23187 at: (B) 0.5 μM, (C) 1 μM, (D) 5 μM, and (E) 10 μM. (F) Spermatozoa treated with 20 μM of A23187 for 10 min and then washed by centrifugation. Hyperactivation was measured at different time points 5, 15, 30, 45, and 60 min. Bars represent average ± SEM. The percentage of hyperactive motile spermatozoa was obtained using CASAnova software (n = 3).