Fig. 7.

PQC proteins co-aggregate with BAG3^P209L. a Fractionation of HEK293 cells expressing Myc-HSPB8 and BAG3 variants. Western blots using the indicated antibodies is shown. The same samples as in Fig. 5e have been used, loading control is therefore the same. b Immunofluorescence pictures of HeLa cells expressing FLAG-BAG3^P209L. Cells were stained with the indicated antibodies for staining endogenous chaperones except HSPB8 (which was stained using antibodies against myc after expression of myc-HSPB8). Scale bar = 5 μm. c Immunofluorescence pictures of HeLa cells expressing BAG3^WT or BAG3^P209L (upper row in green) with V5-DNAJB1^WT or V5-DNAJB1^H32Q (middle row in red). Lower row depicts the merge with DAPI. Scale bar = 5 μm. d Filter trap assay of HEK293 cells expressing HttQ119-YFP together with tetracyclin-inducible DNAJB6b and the indicated FLAG-BAG3 variants. Immunoblot using a GFP antibody is shown. Source data are provided as a Source data file