Table 1.

Primer sequences used for amplification of reference and target genes, amplicon length, amplification temperature (Ta) and melting temperature (Tm).

Function	Gene	Primer Sequence (forward/reverse primer)	Accession number	Matrix	Amplicon length (bp)	Melting temperature (Tm)	Amplification temperature (Ta)
Reference genes	EF1 (EFl-α elongation factor)	5′-GAACTGGGTGCTTGATAGGC -3′/ 5′- AACCAAAATATCCGGAGTAAAAGA-3′	GU585871	Leaves	150	81.5°C
	39SRP (39S Ribosomal protein L41-A)	5′ GACTGACTTCAAGCTTAAACC -3′/ 5′ GATATAACAGGGAATACAGCAC-3′	XM 002285709.1		282	81°C
	ADH2 (Alcohol dehydrogenase 2)	5′-GACCATGTTCTTCCTGTATTCAC-3′/ 5′- GTAGCACCAAGACCTGTAGAG-3′	NM_001281154.1	Green stem	293	82.5°C
	60SRP (60S Ribosomal Protein L18)	5′-ATCTACCTCAAGCTCCTAGTC -3′/ 5′- CAATCTTGTCCTCCTTTCCT -3′	XM 002270599		166	81.5°C
Defense proteins	Glue (β-1,3 glucanase)	5′-TCAATGGCTGCAATGGTGC -3′/ 5′- CGGTCGATGTTGCGAGATTTA-3′	DQ267748	Leaves/green stem	155	81°C
	PR6 (Serine-protease inhibitor 6)	5′-AGGGAACAATCGTTACCCAAG -3′/ 5′- CCGATGGTAGGGACACTGAT-3′	AY156047	Leaves	91	80.5°C
Detoxification and stress tolerance	Hahl (Haloacid dehalogenase hydrolase)	5′-CCCTCAGGATAGCCAACATCA -3′/ 5′- AGGTGCCAACCAGAACTGTGT-3′	NM_001281217	Leaves/green stem	111	81°C	60°C
	HSP (alpha crystalline heat shock protein)	5′-TCGGTGGAGGATGACTTGCT -37 5′- CGTGTGCTGTACGAGCTGAAG-3′	XM_002272382	Leaves/green stem	101	81°C
	GST5 (Glutathione S-transferase 5)	5′-GCAGAAGCTGCCAGTGAAATT-3′/ 5′- GGCAAGCCATGAAAGTGACA-3′	XM 002277883	Leaves	101	79°C
Phenylpropanoid metabolism	STS (Stilbene synthase)	5′-AGGAAGCAGCATTGAAGGCTC -3′/ 5′- TGCACCAGGCATTTCTACACC-3′	FJ851185	Green stem	101	80.5°C
Cell wall compounds	fascAGP (fasciclin-like arabinogalactan protein)	5′-CGAAACCCCAAAGCCTAAGAA -3′/ 5′- GAAAACACAAAGGGGTTGCA-3′	XM 002280793.2	Green stem	101	80.5°C
Aquaporin	PIP2.2 (aquaporin plasma membrane intrinsic protein 2-2)	5′-GGTTCAGTCTCCATTGCACATG -3′/ 5′- TTGGCAGCACAGCAGATGTAT-3′	NM 001280956	Green stem	101	78°