Figure 7.

Validation of a representative subset of mRNA:miRNA pairs involved in the brain aging and neurodegeneration. Relative expression of let-7c-5p, miR-181a-5p, miR-26b-5p, let-7e-5p, miR-29a-3p, miR-146a-5p, and miR-128-3p in the hippocampus of 2 and/or 9 months of age SAMP8 and age-matched SAMR1 mice (A–H). Relative expression of P2rx1, Hmg20b, Pbx1, Nup160, Pou3f2, and Socs6 in the hippocampus of 2- or 9-month-old SAMP8 and age-matched SAMR1 mice (A–H). mRNAs and miRNAs expression was measured by real-time PCR analysis from hippocampal mRNA and small RNA fractions, and expressed relative to U6 snRNA transcript or β-actin levels, respectively (n = 4–5/group). Mean ± standard error from five independent experiments performed in duplicates are represented. Student's T-test results are indicated as ^*p < 0.05; ^**p < 0.01; ^***p < 0.001 and; ^****p < 0.0001.