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. 2018 Dec 11;9:596. doi: 10.3389/fgene.2018.00596

Figure 8.

Figure 8

Brain-Deprived Neurotrophic Factor dysregulation in the hippocampus of 9-month-old SAMP8 mice. Validation of hippocampal miR-191 expression in 9-month-old SAMP8 and age-matched SAMR1 mice (A). Correlation between BDNF protein levels and miR-191 gene expression (B). Representative Western blot (Wb) for determination of BDNF protein levels in 9-month-old SAMP8 and age-matched SAMR1 mice (C), and quantification (D). MicroRNA expression was measured by real-time PCR analysis from hippocampal small RNAs fraction and expressed relative to U6 snRNA transcript levels (n = 4–5/group). Spearman correlation was performed between protein levels of BDNF and miR-191 gene expression in both groups (n = 16). R and p-value were indicated on graphs. Mean ± standard error from three independent experiments performed in duplicates is represented; Values in Wb bar graphs are adjusted to 100% for protein levels of 9-month-old SAMR1. Student's T-test results are indicated as *p < 0.05 and ****p < 0.001.