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. 2018 Dec 11;9:1406. doi: 10.3389/fphar.2018.01406

FIGURE 2.

FIGURE 2

Comparative histochemical and immunohistochemical analysis for ASM, F4/80, Ki67 expression and neovascularization in 5-Ht1a receptor KO and Wild-type mice (excisional punch biopsy model). Dermal fibroblasts in wound area showed non-significant ASM expression in both group WT and KO, (A,B,E). Macrophage count (percentage of F4/80 +ve cells) is high in the wound area of the KO group as compared to WT (C,D,F). Trichrome staining showed mean number of blood vessels in wound area of the KO mice group is lower than the WT mice group (G–I). Expression of Ki67 +ve cell percentage is higher in the KO mice group as compared to WT (J–L) (n = 4 images per specimen). Arrow indicates cells expressing markers and arrow heads indicate absence of marker expression. All images were presented at 50 μm scale bar. Results presented as mean ± 95 CI (confidence interval). Two-way ANOVA was performed and significance levels were set at ∗P < 0.05, ∗∗P < 0.01.