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. 2018 Dec 11;9:1406. doi: 10.3389/fphar.2018.01406

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Copyright © 2018 Sadiq, Menchetti, Shah, Jeschke, Belo, Carlos-Alcalde, Hayat and Amini-Nik.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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In vitro comparative study for cell viability, apoptosis, proliferation, and migration between 5-Ht1a receptor Knock out and Wild-type mouse fibroblast cultures. Comparative proliferation (A,B,G), viability (C) and apoptosis (D) in KO and WT fibroblasts (n = 5 images per sample). Migration assay staining with DAPI and Phalloidin (E,F). Scale bar represents the image magnification. Comparative fibroblast migration (KO vs. WT) was measured in term of width of the scratch zone (μm) from Time 0 to Time 18 h (H). Graphical results were presented as mean ± 95 CI (confidence interval). All experiments were performed three times. Two-way ANOVA was performed and significance levels were set at ^∗P < 0.05, ^∗∗P < 0.01.