Table V.
Comparison of quantitative polymerase chain reaction (qPCR) and sequencing results.
| qPCR results | |||||
|---|---|---|---|---|---|
| Gene | Day 0 | Day 7 | qPCR results Log2 fold change | RNA-seq results Log2 fold change | Up/down |
| lnc_1893 | 1.01±0.162 | 0.04±0.010 | −4.658356 | −6.134612028 | Down |
| lnc_1090 | 1.01±0.141 | 0.31±0.132 | −1.704159 | −5.851519656 | Down |
| lnc_1708 | 1.06±0.362 | 0.15±0.013 | −2.821126 | Nam | Down |
| lnc_709 | 1.05±0.327 | 0.12±0.024 | −3.129103 | Nam | Down |
| lnc_1369 | 1.07±0.405 | 2.70±0.446 | 1.335369 | Inf | Up |
| lnc_554 | 1.15±0.613 | 2.98±0.218 | 1.373676 | Inf | Up |
| lnc_1269 | 1.03±0.233 | 5.03±0.547 | 2.287915 | Inf | Up |
| lnc_1307 | 1.04±0.290 | 31.00±4.245 | 4.897613 | Inf | Up |
| ENSG00000238042 | 1.10±0.475 | 0.12±0.019 | −3.196277 | −3.088732191 | Down |
| ENSG00000250049 | 1.04±0.286 | 2.99±0.852 | 1.523562 | 2.529276235 | Up |
| ENSG00000224750 | 1.04±0.272 | 0.34±0.082 | −1.613079 | −6.630336214 | Down |
| ENSG00000238266 | 1.05±0.304 | 2.93±0.681 | 1.480524 | 2.308285071 | Up |
‘Inf’ indicates that the expression level in Day 0 group was 0; compared with that in the Day 0 group, the expression in the Day 7 group was greatly upregulated. ‘Nam’ indicates that the expression level in the Day 7 group was 0; compared with that in the Day 0 group, the expression in the day 7 group was greatly downregulated. GAPDH was used as the internal control. The results are expressed as the mean ± standard deviation. qPCR, quantitative polymerase chain reaction; RNA-seq, RNA-sequencing.