Figure 2.

HIF-1α deficiency decreases apoptosis and the expression levels of apoptosis-associated proteins in GC-2 cells. HIF-1α-deficient GC-2 cells and control cells were subjected to hypoxic conditions for 48 h. (A) Cell apoptosis was detected by flow cytometry analysis. (B) Reverse transcription-quantitative polymerase chain reaction analysis detected miR-210 expression in GC-2 cells. (C) HIF-1α and apoptosis-associated proteins were evaluated by western blot analysis. **P<0.01 vs. respective si-Ctrl. HIF-1α, hypoxia-inducible factor-1α; miR, microRNA; si, small interfering; Ctrl, control; PI, propidium iodide; Bax, apoptosis regulator BAX; Bcl-2, B-cell lymphoma 2; GC-2, GC-2spd.