Figure 4.

miR-210 target prediction. (A) Interaction between miR-210 and the 3′UTR of KLF7 was predicted using TargetScan (red letters indicate the binding sites), and luciferase activity was assessed by a luciferase assay. **P<0.01 vs. mimics NC. (B) miR-210 effect on KLF7 expression. **P<0.01 vs. mimics NC; ^##P<0.01 vs. inhibitor NC. (C) KLF7 protein expression level in GC-2spd cells under hypoxic culture conditions. **P<0.01 vs. normal. miR, microRNA; KLF7, Kruppel-like factor 7; 3′UTR, 3′untranslated region; Wt, wild-type; Mut, mutant; NC, negative control.