Figure 3.

Decreased expression of PTEN in the Tet1 interference group is associated with its increased methylation. (A and B) HGC-27 cells were infected with viruses carrying a Tet1 interference vector. mRNA and protein expression levels of PTEN were detected in the cells using reverse transcription-quantitative PCR or western blotting, respectively. (C) The methylation status of PTEN was detected using methylation-specific PCR. ***P<0.001 vs. the NC group (n=3). M, methylated; NC, negative control; PCR, polymerase chain reaction; PTEN, phosphatase and tensin homolog; Tet1, tet methylcytosine dioxygenase 1; U, unmethylated.