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. 2018 Dec 11;9:2824. doi: 10.3389/fimmu.2018.02824

Figure 2.

Figure 2

DWEYS inhibits the transfer of the interferon signature by SLE serum but is not a TLR9 antagonist. (A) Primary human monocytes were incubated with SLE serum with or without DWEYS or with control peptide for 4 h. lSG expression was evaluated by qPCR. Results indicate mean ± SD of three independent experiments. (B) Assessment of DWEYS and CpG oligonucleotides binding to TLR9. Labeled TLR9 was incubated with varying concentrations of CpG or DWEYS for 30 min in room temperature. Binding was assessed using an AlphaScreen assay as described in the methods section. (C,D) Inhibition assay. Labeled TLR9 and constant, binding concentration of CpG were incubated with different concentrations of DWEYS or a control peptide at neutral (pH 7.4, C) or acidic (pH 6.25, D) condition. Binding was assessed using an AlphaScreen assay as noted in the methods section. Results are representative of three independent experiments. ***p < 0.001, ns, not significant.