Figure 2.

Puerarin promotes cell apoptosis and inhibits cell viability of breast cancer. (A) HCC38 cell apoptotic rate following treatment with 20 µM puerarin, miR-133a-3p mimics, 20 µM puerarin + miR-133a-3p mimics and NC group detected by a flow cytometry assay. *P<0.05, **P<0.01 vs. NC. (B) Apoptosis rate expressed as a percentage. (C) Cell viability following the treatment with puerarin at various concentrations at 24, 48 and 72 h, detected by a CCK-8 assay. *P<0.05, **P<0.01 vs. 24 h. (D) Cell viability detected using a CCK-8 assay in four treatment groups. **P<0.01 vs. NC. NC, negative control; CCK-8, Cell Counting Kit-8; miR, microRNA; PI, propidium iodide; FITC, fluorescein isothiocyanate.