Figure 2.

Lymphocytes are not required for the exercise-induced increase in hippocampal precursor proliferation. (A) Experimental design. Following exercise, a significant increase in the number of proliferating precursor cells was observed within the DG of C57BL/6 (B), Dereg (C), TCRα^−/− (D), Rag1^−/− (E), and Rag2^−/−γc^−/− (F) mice. (G) Representative image of BrdU staining in the DG of Rag2^−/−γc^−/− mice. No change in baseline proliferation, or a response to physical exercise was observed in the other neurogenic niche, the SVZ, in C57BL/6 (H), TCRα^−/− (I), Rag1^−/− (J), and Rag2^−/−cγ^−/− (K) mice. (L) Representative image of BrdU staining in the DG of Rag2^−/−cγ^−/− mice. Symbols and horizontal lines indicate individual mice and mean values ± SEM, respectively. The level of significance was determined using a two-tailed Student's t-test. ^*p < 0.05, n.s = not significant. Scale bars in (F,K) are 50 μm.