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. 2018 Jul 27;315(5):H1293–H1303. doi: 10.1152/ajpheart.00213.2018

Table 2.

Primers used for DNA methylation-specific quantitative PCR

Gene Species Sequence
vWF Homo sapiens Forward: 5′-TTTCGAGTAGTTGGGATTATAGGC-3′
Reverse: 5′-TAAAAAAAACCATTAAAAAACCGAA-3′
Mus musculus Forward: 5′-ATTGTTTTTTGTTATTGTTTTTCGT-3′
Reverse: 5′-AAATCTACTCAAACTCTAAACCGAC-3′
CD31 Homo sapiens Forward: 5′-GGATTATAGGCGTGAGTTATTGC-3′
Reverse: 5′-AAATTATAAAATTCCCGAATTTCGT-3′
Mus musculus Forward: 5′-TTATAGAAAGAAGTTGTTTGTTTCGG-3′
Reverse: 5′-TCTACAATCTTTACGACACATCGAT-3′
VE-cadherin Homo sapiens Forward: 5′-GGTACGTAGATTATGAGGATAGGC-3′
Reverse: 5′-AAAATAAACAAACAACAACTCAACG-3′
Mus musculus Forward: 5′-TGGTTTGAAGGTTATTTTAGAGACG-3′
Reverse: 5′-CTACCAAAAAAACAAAAAACACGAT-3′
α-SMA Homo sapiens Forward: 5′-TTTACGTTTATTTTAACGTGGAGC-3′
Reverse: 5′-ACCTAAAAACGACGAACTACTACGA-3′
Mus musculus Forward: 5′-TTTTTTAAAGGAATTGTTAGTTCGT-3′
Reverse: 5′-CAATAAATCCTTATCATAAACATCGTA-3′
N-cadherin Homo sapiens Forward: 5′-GTGCGAGTTTTAGAGAGGAGTC-3′
Reverse: 5′-TATCTCCTTAAAAACCTAACGTACG-3′
Mus musculus Forward: 5′-GGATTTAATATAGGGTTAGGGTCGT-3′
Reverse: 5′-ATAAAAAACCACCGAACTACGAA-3′
FSP1 Homo sapiens Forward: 5′-GTTTTTGAGATGTGGGTTTGTATAC-3′
Reverse: 5′-CCTTCTCGAACTAAACTACTACGTA-3′
Mus musculus Forward: 5′-GTTTCGGGATTTTAGAGATTTTAGC-3′
Reverse: 5′-CAAACCTAATAAACATAACTTCGAA-3′
Vimentin Homo sapiens Forward: 5′-GTATGAATTGGTGGATTTTGTAGAC-3′
Reverse: 5′-TTATACACTCTAAAACCCTAAACGAA-3′
Mus musculus Forward: 5′-GAGTTTTTGGAACGTTAGATGC-3′
Reverse: 5′-TTCTAAATCTCATCCTACAAACGAC-3′
UBB Homo sapiens Forward: 5′-ATAGTGGGTTTTGTTGATTTGA-3′
Reverse: 5′-CCTTTCTCACACTAAAATTCCA-3′
Mus musculus Forward: 5′-GATTTAGTAGGTTTTTAAATTTTTATT-3′
Reverse: 5′-ATACCCTCTTTATCCTAAATCTTAA-3′

vWF, von Willebrand factor; VE-cadherin, vascular-endothelial cadherin; α-SMA, α-smooth muscle actin; N-cadherin, neural cadherin; FSP1, fibroblast-specific protein 1; UBB, ubiquitin B.