Fig. 3.

Cardiac myocyte terminal differentiation and cell cycle activity. A: proportionally fewer myocytes were binucleate with early testosterone propionate (early T) treatment compared with vehicle (control) or late testosterone propionate (late T) treatment, fewer myocytes were binucleate in male than female fetuses, and fewer myocytes were binucleate in the left ventricle (LV) than in the right ventricle (RV) in fetal sheep at ~135 days of gestation after maternal exposure to control (n = 8 female fetuses and 4 male fetuses) or early T (n = 4 female fetuses and 5 male fetuses) or late T (n = 5 female fetuses and 5 male fetuses). B: cell cycle activity was less in early T than control or late T and less in the LV than in the RV. Values are means ± SE. Comparisons were performed by three-way mixed-model ANOVA. *Different from control and late T; †different from female fetuses; ‡different from the RV (P < 0.05, exact values in text).