Table 6.
Plasma cytokine levels in OVX female rats with or without E2 replacement at 10 wk after MI
| Cytokine, pg/ml | Sham OVX-sham MI Group (n = 6) | OVX-Sham MI Group (n = 7) | OVX-E2-Sham MI Group (n = 7) | OVX-MI Group (n = 10) | OVX-E2-MI Group (n = 9) |
|---|---|---|---|---|---|
| IL-1α | 74 ± 9.8 | 70 ± 9.1 | 52 ± 11.3 | 82 ± 9.8 | 104 ± 19.9 |
| IL-1β | 144 ± 46.1 | 213 ± 27.5 | 399 ± 86.5 | 135 ± 20.3 | 207 ± 60.1 |
| IL-2 | 123 ± 16.4 | 131 ± 65.4 | 82 ± 9.0 | 193 ± 27.3* | 138 ± 9.8* |
| IL-6 | 479 ± 85.1 | 439 ± 109.3 | 357 ± 82.0 | 928 ± 173.6* | 630 ± 54.2* |
| IL-10 | 107 ± 26.2 | 82 ± 19.6 | 33 ± 5.9 | 131 ± 24.2* | 203 ± 34.9*† |
| TNF-α | 213 ± 49.1 | 149 ± 39.7 | 76 ± 6.9 | 288 ± 53.8* | 396 ± 64.4* |
Values are means ± SE; n, number of rats/group. E2, 17β-estradiol. One-way ANOVA was done to compare all groups, and two-way ANOVA was performed for the ovariectomy (OVX) and myocardial infarction (MI) interaction followed by a Bonferroni post hoc test. OVX × MI interaction for IL-10: F = 5.4, P = 0.027. MI effect: TNF-α, F = 19.7, P < 0.0001; IL-6, F = 5.0 P = 0.023; and IL-2, F = 5.1, P = 0.033. There was no MI or OVX effect for IL-1α or IL-1β.
*
P < 0.05 vs. the related sham MI group;
†
P < 0.05 vs. the OVX-MI group.