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. 2018 Dec 11;9:2838. doi: 10.3389/fimmu.2018.02838

Figure 2.

Figure 2

Correlation between bacterial metabolites production and IDO-1 gene expression. (A) Effect of bacterial supernatants on IDO-1 reporter system organized by phylum. Culture supernatants of a wide range of cultivable commensal bacteria were applied on the HT-29-IDO-1 reporter system (10% vol/vol) for 24 h. IDO-1 expression was measured by luciferase activity and expressed as fold increase toward its control: non inoculated growth medium used for each culture. IDO-1 expression profiles upper and lower the dash lines were considered as significantly changed. (B) PCA analysis showing the correlation between the SCFAs concentrations produced by the commensal bacteria and IDO-1 expression. (C,D) Representation of IDO-1 expression correlated to butyrate (C) and acetate (D) concentration in in bacterial cultures classified by rank value. Actinobacteria in blue, Bacteroidetes in yellow, Firmicutes in gray, Fusobacteria in red and Verrucomicrobiea in light blue.