Table 2.
RT-qPCR markers.
| Gene | Forward primer sequence 5′-3′ | Reverse primer sequence 5′-3′ |
|---|---|---|
| BRAIN | ||
| Ccl2 | CAAGATGATCCCAATGAGTAG | TTGGTGACAAAAACTACAGC |
| Ccl4 | GGTATTCCTGACCAAAAGAG | TCCAAGTCACTCATGTACTC |
| Ccl12 | CAGTCCTCAGGTATTGGCTGGA | TCCTTGGGGTCAGCACAGAT |
| Il-1β | TGTAATGAAAGACGGCACACC | TCTTCTTTGGGTATTGCTTGG |
| Tnf-α | CCCTCACACTCAGATCATCTTCT | GCTACGACGTGGGCTACAG |
| Il-6 | GAGCCCACCAAGAACGATAG | GGTTGTCACCAGCATCAGTC |
| Cd36 | GGACATTGAGATTCTTTTCCTCTG | GCAAAGGCATTGGCTGGAAGAAC |
| Il-4rα | CAGATCCCAGATACTCACTG | AGTAACAGAACAGGCAAAAC |
| Tgf-β1 | TGATACGCCTGAGTGGCTGTCT | CACAAGAGCAGTGAGCGCTGAA |
| Gapdh | ATGACATCAAGAAGGTGGTG | CATACCAGGAAATGAGCTTG |
| MUSCLE | ||
| G-csfr | ACAAAGCAGGGACCTCTTCA | ATGGTGTTAAGGTCTTGGGC |
| Tgf-β | ATTCCTGGCGTTACCTTGG | CCTGTATTCCGTCTCCTTGG |
| Ccl2 | CAAGATGATCCCAATGAGTAG | TTGGTGACAAAAACTACAGC |
| Ccl4 | GGTATTCCTGACCAAAAGAG | TCCAAGTCACTCATGTACTC |
| Tnf-α | CCCTCACACTCAGATCATCTTCT | GCTACGACGTGGGCTACAG |
| Il-1β | TCCATGAGCTTTGTACAAGG | GGTGCTGATGTACCAGTTGG |
| β2-mg | TTCACCCCCACTGAGACTGAT | GTCTTGGGGTCGGCCATA |
Inflammatory genes of interest for brain and muscle tissue analysis included chemokine (C-C motif) ligand (Ccl)2, Ccl4, Ccl12, interleukin-1β (Il-1β), Il-6, tumor necrosis factor-α (Tnf-α), Cd36, IL-4 receptor α (Il-4rα), granulocyte colony-stimulating factor receptor (G-csfr), and transforming growth factor-β1 (Tgf-β1). Glyceraldehyde 3-phosphate dehydrogenase (Gapdh) and β2-microglobulin (β2-mg) served as housekeeping genes.