Figure 2.

Deletions in Dll-Coding Region Generate Leg and Wing Mutants

(A–C) (A) The Homeodomain (Exon 3) and upstream coding exon (Exon 2) of Dll were targeted using CRISPR/Cas9. Three guides were designed: two within the coding regions of Exon 2 and one within the coding region of Exon 3. (B) Mosaic mutant with a disrupted wing margin (Exon 2, sgRNA-2B mutant). (C) Mosaic mutant males with leg deformations (Leg M1 and M2: Exon 2 sgRNA-2B mutants, Leg M3: Exon 3 mutant)