Figure 4.

Ectopic Sternite Brush Phenotypes Obtained with Genome Editing of Exon 2

(A–D) (A) Ectopic mutant with ectopic brush on third ventral abdominal segment (E2BM1). (B and C) Ectopic mutants with ectopic brushes on the fourth dorsal abdominal segment (E2BM2 and E2BM3). (D) Sequences confirm that mutations underlying the ectopic brush phenotypes (E2BM1, E2BM2, E2BM3) lie within a predicted exonic splicing enhancer (ESE), whereas mutations underlying sternite malformation phenotypes (see Table S1) lie outside of the predicted ESE. The alignment is reverse complemented to display the putative ESE site sequence as predicted by ESEfinder 3.0 (Cartegni et al., 2003).