Figure 5.

CRISPR/Cas9-Induced Deletions in Exonic Splicing Enhancer (ESE) of Exon 2 (sgRNA-2B) Generate Exon-Skipped Dll Transcripts

(A) Primers flanking sgRNA-2B were used to amplify cDNA obtained from the two mosaic mutant embryo replicates (C1 and C2) and wild-type embryos. C1 and C2 mosaic mutants produced a mixture of a short PCR product (∼500 bp) and the wild-type PCR product (726 bp).

(B) Sanger sequencing of the shorter PCR product obtained from C1 and C2 confirms that Exon 2 has been excluded; an altered Dll transcript is present in both replicates of Exon 2 mutant embryos. Sanger sequencing of the longer product yields a wild-type Dll sequence.