Figure 6.

Protein Predictions

(A) The exon-skipped Dll transcript generates an altered protein with a disrupted homeodomain. However, with leaky scanning, the exon-skipped Dll transcript can generate an altered protein that recapitulates the wild-type protein sequence from Exon 3 onward with an intact homeodomain. Analysis with a TripleTOF 5600 recovered peptides that matched to the predicted “Dll protein under leaky scanning.”

(B) The in vitro-synthesized truncated Dll protein and control reaction were separated on a 10% SDS-polyacrylamide gel and visualized with Coomassie blue staining. The exon-skipped Dll transcript was translated into a >30-kDa protein product.