Figure 7.

Mutant Phenotypes Obtained with Genome Editing of Second Putative ESE Site in Exon 2 (sgRNA-2A)

(A–C) (A) Mutant with ectopic brush on fourth ventral abdominal segment (E2A M1). (B) Mutant with loss of half of the fourth sternite brush (E2A M2). (C) Sequences confirm that mutations underlying the ectopic brush phenotype (E2A M1) lie within a predicted exonic splicing enhancer (ESE), whereas mutations underlying sternite malformation phenotypes lie outside of the predicted ESE. The two most abundant mutant haplotypes for E2A M1 are shown in this alignment (E2A M1a is a single-point deletion, whereas E2A M1b is a 57-bp deletion that disrupts this target ESE site as well as another downstream ESE site).