Table 1.
Injected Eggs | Larvae | Adults | Mutants |
||||
---|---|---|---|---|---|---|---|
Leg/Wing | Sternite Malformations | Ectopic Structures | Others (e.g., Clasper Malformations) | ||||
Exon 2 (sgRNA 2A) | 1,715 | 605 | 151 | 1 | 8 | 1 | 0 |
Exon 2 (sgRNA 2B) | 4,631 | 957 | 363 | 8 | 29 | 3 | 6 |
Exon 3 (sgRNA3) | 4,003 | 1,187 | 261 | 1 | 21 | 0 | 2 |
Control | 1,004 | 153 | 109 | 0 | 0 | 0 | 0 |
Control injections were carried out using only Cas9 protein without any sgRNA.