Table 3.
Morphological classes and morphotypes of MPh on the 6th day of cultivation on standard culture plastics and biopolymer scaffolds of various composition. MN were isolated from the patient's blood before the stent was installed and after the stent was installed. MN were cultivated on culture plastic and on biopolymers scaffolds of various compositions (scaffolds 1, 2, 3, 4, 5) in 96-well culture plates (105 cells/well) in DMEM medium with 10% fetal serum in CO2-incubator. After 6 days of culture the cells were fixed in the wells of the plate with 2.5% glutaraldehyde and samples were prepared for SEM using a standard protocol.
| Experimental condition |
Morphological classes, % of total cell number |
||||||
|---|---|---|---|---|---|---|---|
| Morphological class 1 Rounded cells |
Morphological class 2 Elongated cells |
Morphological class 3 Cells with unusual morphology |
|||||
| Morphotype 1 Multinuclear cells |
Morphotype 2 Mononuclear cells |
Morphotype 1 Filiform cells |
Morphotype 2 Spindle-shaped cells |
Morphotype 3 Rod-shaped cells |
Morphotype 4 «Triangular» cells |
||
| Culture plastic, before stenting |
19.75 | 28.18 | 3.50 | 26.75 | 5.73 | 15.13 | 0.96 |
| Culture plastic, after stenting |
19.10 | 35.76 | 1.04* | 30.90 | 9.72* | 3.13* | 0.35* |
| Scaffold 1, before stenting |
15.98 | 18.14 | 6.26 | 43.20 | 9.94 | 2.59 | 3.89 |
| Scaffold 1, after stenting |
20.49 | 22.97 | 9.19* | 33.22 | 6.01* | 1.77* | 6.36* |
| Scaffold 2, before stenting |
10.87 | 54.35 | 1.09 | 23.91* | 3.26 | 3.26 | 3.26 |
| Scaffold 2, after stenting |
11.51 | 9.47* | 17.99* | 50.00 | 6.24* | 0 | 2.52 |
| Scaffold 3 before stenting |
9.30 | 46.51 | 3.10 | 30.23 | 5.43 | 4.65 | 0.78 |
| Scaffold 3 after stenting |
4.17* | 37.50 | 4,.17 | 41.67 | 8.33* | 4.17 | 0.00 |
| Scaffold 4 before stenting |
14.98 | 20.70* | 8.81 | 36.56 | 10.57 | 6.17 | 2.20 |
| Scaffold 4 after stenting |
5.45* | 13.64 | 3.64* | 60.91* | 6.36* | 7.27 | 2.73 |
| Scaffold 5 before stenting |
10.48 | 31.43 | 3.81 | 37.14 | 11.43 | 5.71 | 10.48 |
| Scaffold 5 after stenting |
17.74 | 40.32 | 4.84 | 29.03 | 3.23* | 3.23* | 1.61* |
An asterisk indicates the cases where the value after the stent installation (before stenting) was significantly different from the value before the stent installation (after stenting) (p ≤ 0.05).