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. 2018 Nov 16;6:74–90. doi: 10.1016/j.toxrep.2018.11.009

Table 3.

Morphological classes and morphotypes of MPh on the 6th day of cultivation on standard culture plastics and biopolymer scaffolds of various composition. MN were isolated from the patient's blood before the stent was installed and after the stent was installed. MN were cultivated on culture plastic and on biopolymers scaffolds of various compositions (scaffolds 1, 2, 3, 4, 5) in 96-well culture plates (105 cells/well) in DMEM medium with 10% fetal serum in CO2-incubator. After 6 days of culture the cells were fixed in the wells of the plate with 2.5% glutaraldehyde and samples were prepared for SEM using a standard protocol.

Experimental
condition
Morphological classes, % of total cell number
Morphological class 1
Rounded cells
Morphological class 2
Elongated cells
Morphological class 3
Cells with unusual morphology
Morphotype 1
Multinuclear
cells
Morphotype 2
Mononuclear
cells
Morphotype 1
Filiform cells
Morphotype 2
Spindle-shaped cells
Morphotype 3
Rod-shaped cells
Morphotype 4
«Triangular» cells
Culture plastic,
before stenting
19.75 28.18 3.50 26.75 5.73 15.13 0.96
Culture plastic,
after stenting
19.10 35.76 1.04* 30.90 9.72* 3.13* 0.35*
Scaffold 1,
before stenting
15.98 18.14 6.26 43.20 9.94 2.59 3.89
Scaffold 1,
after stenting
20.49 22.97 9.19* 33.22 6.01* 1.77* 6.36*
Scaffold 2,
before stenting
10.87 54.35 1.09 23.91* 3.26 3.26 3.26
Scaffold 2,
after stenting
11.51 9.47* 17.99* 50.00 6.24* 0 2.52
Scaffold 3
before stenting
9.30 46.51 3.10 30.23 5.43 4.65 0.78
Scaffold 3
after stenting
4.17* 37.50 4,.17 41.67 8.33* 4.17 0.00
Scaffold 4
before stenting
14.98 20.70* 8.81 36.56 10.57 6.17 2.20
Scaffold 4
after stenting
5.45* 13.64 3.64* 60.91* 6.36* 7.27 2.73
Scaffold 5
before stenting
10.48 31.43 3.81 37.14 11.43 5.71 10.48
Scaffold 5
after stenting
17.74 40.32 4.84 29.03 3.23* 3.23* 1.61*

An asterisk indicates the cases where the value after the stent installation (before stenting) was significantly different from the value before the stent installation (after stenting) (p ≤ 0.05).