Table 4.
MTT assay: activity of MTT reduction to formazan in various cultivation conditions. MN were isolated from the patient's blood before the stent was installed (Before) and after the stent was installed (After). MN were cultured on culture plastic (CP) and on the biopolymer scaffolds of various compositions (S1, S2, S3, S4, S5) in 96-well culture plates (105 cells/well) in DMEM medium with 10% fetal serum in a CO2 incubator. After 6 days of culture the activity of MTT reduction to formazan was determined. MTT assay results were presented in % of control (culture plastic, 100%). The activity of MTT reduction is presented as an optical density at λ = 550 nm in parentheses, under percentages.
| MN isolation | Scaffolds |
|||||
|---|---|---|---|---|---|---|
| S1 | S2 | S3 | S4 | S5 | CP | |
| Before | 116% (0.405) |
63% (0.220)* |
68% (0.237)* |
98% (0.338) |
82% (0.284) |
100% (0.348) |
| After | 118% (0.232)# |
92% (0.180) |
119% (0.233) |
149% (0.293)* |
109% (0.214) |
100% (0.196)# |
Asterisk * indicates the values significantly different from the values for culture plastic (CP) (p ≤ 0.05) for each variant of MN isolation (before or after stenting). The # icon indicates the values for the MN isolated after the stent installation (After) significantly different from the values for the MN isolated before the stent installation (Before) (p ≤ 0.05).