Fig. 4.

Stable Expression of both A3C-Ile188 and A3C-Ser188 in SupT11 cells provides a partial block to Vif-deficient HIV-1 replication. a Flow cytometry plots for SupT11 cells 72 h after transduction with the indicated GFP-marked complementation vectors. The percentage of GFP+ cells is indicated for each condition. b Immunoblots of A3C in SupT11 cells transduced with constructs expressing A3C-Ser188, A3C-Ile188, or an empty control vector. Tubulin was used as a loading control. c Representative spreading infection data for Vif-deficient HIV-1 (MOI = 0.01) in SupT11 cells expressing A3C-Ser188, A3C-Ile188, or an empty control vector. Virus infectivity was determined by infection of CEM-GFP with culture supernatants followed 48 h later by quantification with flow cytometry. These data are from one of four biologically independent experiments. d Average G-to-A mutation loads for each condition. Error bars show ± SD of 4 independent experiments. Statistical comparisons were done using Student’s t test. p values above each panel are in comparison to vector control or A3C-Ser188. e Mutation data for each condition