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. 2018 Dec 17;6:226. doi: 10.1186/s40168-018-0605-2

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© The Author(s). 2018

Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 6 — Diagnosing contamination in an exploratory analysis of the vaginal microbiota and preterm birth (PTB). The association between PTB and an increase in the average gestational frequency of various ASVs was evaluated in the two cohorts of women (Stanford and UAB) analyzed in [19]. The x- and y-axes display the P values of the association between increased gestational frequency and PTB (one-sided Wilcoxon rank-sum test) in the Stanford and UAB cohorts, respectively. Points are colored by the score assigned to them by the isContaminant function in the decontam R package, using the combined method. Several ASVs that were strongly associated with PTB in either the Stanford or UAB cohorts are clearly identified as contaminants with a decontam score of less than 10⁻⁵ (genera in magenta text)