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. Author manuscript; available in PMC: 2018 Dec 18.
Published in final edited form as: Nano Lett. 2018 Sep 19;18(10):6500–6508. doi: 10.1021/acs.nanolett.8b03032

Figure 1.

Figure 1.

MWCNTs induce focus formation and collagen production in primary human lung fibroblasts. (A) Proliferation of NHLFs grown in a standard 2D cell culture. The cells were treated with various concentrations of MWCNT and cell proliferation was determined by MTS assay at 1 and 3 days post-treatment. (B) Immunoblotting of collagen in MWCNT-treated cells. (C) Representative images of focus formation in cells treated with 0.16 μg/cm2 MWCNT. Fibroblast foci were formed under a low seeding density of 2.5 × 103 cells/well in 6-well plates. Images were taken at 14 days post-treatment. (D) Dose dependent effect of MWCNT on focus formation. (E,F) Immunoblotting of collagen production in MWCNT-treated focus-forming cells. (G) Detection of collagen production by Sircol assay in focus-forming cells. *p < 0.05, **p < 0.001 versus Ctrl, n = 4.