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. Author manuscript; available in PMC: 2018 Dec 18.
Published in final edited form as: Nano Lett. 2018 Sep 19;18(10):6500–6508. doi: 10.1021/acs.nanolett.8b03032

Figure 2.

Figure 2.

MWCNTs induce stem-like cells in NHLFs. (A) The 3D spheres formed by NHLFs treated with 0.16 μg/cm2 MWCNT for 14 days under nonadherent conditions. Scale bar is 100 μm. (B) Quantitation of 3D spheres with size larger than 20 μm in diameter. (C) Flow cytometry graphical representation of ALDH activity in MWCNT-treated cells with or without DEAB, an ALDH inhibitor, which was used as an internal control for baseline ALDH activity measurements. (D) Quantification of ALDH activity, means ± s.d. (n = 3), *p < 0.05. (E,F) Immunofluorescence staining and quantification of αSMA and ABCG2 in MWCNT-induced fibroblast foci. Scale bar is 100 μm. (G,H) Immunofluorescence staining and quantification of CD90 and ALDH in MWCNT-induced fibroblast foci using image analysis software. Scale bar is 100 μm. *p < 0.05, **p < 0.001 versus Ctrl, n = 3.