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. Author manuscript; available in PMC: 2019 Dec 13.
Published in final edited form as: Cell. 2018 Dec 13;175(7):1756–1768.e17. doi: 10.1016/j.cell.2018.10.025

Figure 4. Irisin stimulates a very potent pathway of “integrin-like” signaling including pFAK, pZyxin and pCREB.

Figure 4.

(A) Scheme of crosslinking/co-immunoprecipitation/mass spectrometry experiments to identify irisin receptors. (B) Top 5 enriched proteins with irisin versus adipsin. See also Table S4 for full list. (C) Model of canonical integrin signaling. Integrin heterodimer binds to its ligand. The interaction results in phosphorylation of FAK and Zyxin, followed by phosphorylation of AKT (at T308) and CREB. PM is plasma membrane. (D-E) MLO-Y4 cells were seeded and incubated until 60% cell density. The cells were incubated with FreeStyle293 medium for 4 hours and were treated for indicated time with 10nM norepinephrine or irisin (D) or indicated concentrations of irisin for 10 minutes (E). Cells were lysed to detect the indicated protein level using immunoblot analysis.