Fig. 2.

OsSPK1 contributes to Pit-mediated resistance in rice immunity. (A) Transcript levels of OsSPK1 were measured by qPCR in OsSPK1 RNAi plants. Numbers 1, 17, and 32 indicate independent RNAi transgenic lines. OsUbq was used as an internal control. Bars are SE (**P < 0.01; n = 3). (B–D) Infection assays of OsSPK1 RNAi plants with the incompatible M. oryzae race 007.0. (B) Photographs show typical phenotypes of OsSPK1 RNAi plants after infection at 7 dpi. (Scale bar, 5 mm.) (C) Quantitative analysis of lesions induced by the blast fungus was performed at 7 dpi. Relative lesion length (WT = 1) is shown. Error bars indicate SE (*P < 0.05; n ≥ 48). (D) Growth of the incompatible M. oryzae race in OsSPK1 RNAi plants was measured by qPCR. Relative infection ratio (WT = 1) is shown. Error bars indicate SE (**P < 0.01; n ≥ 8). (E and F) Infection assays of OsRacGEF1 RNAi plants with the incompatible M. oryzae race 007.0. (E) Quantitative analysis of lesions induced by the blast fungus was performed at 7 dpi. Relative lesion length (WT = 1) is shown. Error bars indicate SE (**P < 0.01; n ≥ 48). (F) Growth of the incompatible M. oryzae race in OsSPK1 and OsRacGEF1 RNAi plants was measured by qPCR and normalized with endogenous OsUbq. Relative infection ratio (WT = 1) is shown. Error bars indicate SE (**P < 0.01; n ≥ 8). (G) Transcript levels of OsSPK1 were measured by qPCR in OsSPK1 RNAi suspension cells and normalized with endogenous OsUbq expression. Numbers 17 and 32 indicate independent RNAi transgenic lines. Bars are SE (**P < 0.01; n = 3). (H) Cell death activity induced by Pit in OsSPK1 RNAi rice protoplasts. Relative luciferase activity (GUS = 100) is shown. Bars are SE (**P < 0.01, n = 6).