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Sir2–4 complex binds both WT and H4K16Q arrays. (A) EMSA of Sir2–4 binding to WT and H4K16Q arrays. Sir2–4 titrations contain molar ratios of 0, 1, 2, 3, or 4 Sir2–4 per nucleosome. (B and C) vHW plots of Sir2–4 complex titrated onto WT and H4K16Q arrays. Numbers indicate the molar ratio of Sir2–4 complex per nucleosome.
