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. 2018 Dec 11;9:1377. doi: 10.3389/fphar.2018.01377

Table 1.

Comparisons the validation parameters of UPLC-QDA with HPLC-ELSD for analysis of astragaloside IV.

UPLC-QDA HPLC-ELSD
Retention time (min) 6.15 23.75
Linear Y = 29.215X + 0.2772, r = 0.999 Ln(Y) = 1.535ln(X) + 0.7998, r = 0.998
Linearity range (mg/ml) 0.008–0.09 0.27–0.54
LOD (ng) 8 200
LOQ (ng) 25 500
Intra-day RSD (%) (n = 6) 1.96 0.74
Inter-day RSD (%) (n = 3) 1.77 0.21
Repeatability RSD (%) (n = 6) 2.77 1.56
Stability RSD (%) (n = 8) 2.10 1.40
Recovery mean (%) 100.95 (RSD 3.37%) 100.88 (RSD 3.19%)