Table 1.

Comparisons the validation parameters of UPLC-QDA with HPLC-ELSD for analysis of astragaloside IV.

	UPLC-QDA	HPLC-ELSD
Retention time (min)	6.15	23.75
Linear	Y = 29.215X + 0.2772, r = 0.999	Ln(Y) = 1.535ln(X) + 0.7998, r = 0.998
Linearity range (mg/ml)	0.008–0.09	0.27–0.54
LOD (ng)	8	200
LOQ (ng)	25	500
Intra-day RSD (%) (n = 6)	1.96	0.74
Inter-day RSD (%) (n = 3)	1.77	0.21
Repeatability RSD (%) (n = 6)	2.77	1.56
Stability RSD (%) (n = 8)	2.10	1.40
Recovery mean (%)	100.95 (RSD 3.37%)	100.88 (RSD 3.19%)