Table 1.
Comparisons the validation parameters of UPLC-QDA with HPLC-ELSD for analysis of astragaloside IV.
UPLC-QDA | HPLC-ELSD | |
---|---|---|
Retention time (min) | 6.15 | 23.75 |
Linear | Y = 29.215X + 0.2772, r = 0.999 | Ln(Y) = 1.535ln(X) + 0.7998, r = 0.998 |
Linearity range (mg/ml) | 0.008–0.09 | 0.27–0.54 |
LOD (ng) | 8 | 200 |
LOQ (ng) | 25 | 500 |
Intra-day RSD (%) (n = 6) | 1.96 | 0.74 |
Inter-day RSD (%) (n = 3) | 1.77 | 0.21 |
Repeatability RSD (%) (n = 6) | 2.77 | 1.56 |
Stability RSD (%) (n = 8) | 2.10 | 1.40 |
Recovery mean (%) | 100.95 (RSD 3.37%) | 100.88 (RSD 3.19%) |