Table 2.
Multivariable linear regression of smoking behaviours and DNA methylation with top sites from basic model taken forward to models 1–3 after imputation
| Basic model | Adjusted model 1 | Adjusted model 2 | Adjusted model 3 | Adjusted model 4 | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Ever smoked a cigarette reliable (n = 504 ever; n = 435 never) | |||||||||||||||
| Total sample size | 910 | ||||||||||||||
| cg02512902 (KSR1) | −0.016 | −0.023, | 3.64e-06 | −0.014 | −0.021, | 6.36e-05 | −0.012 | −0.019, | 0.002 | −0.011 | −0.018, | 0.003 | −0.011 | −0.019, | 0.002 |
| −0.009 | −0.007 | −0.004 | −0.011 | −0.004 | −0.004 | ||||||||||
| cg19593285 (E2F1) | −0.015 | −0.021, | 1.83e-05 | −0.012 | −0.018, | 1.13e-04 | −0.012 | −0.018, | 3.30e-04 | −0.012 | −0.016, | 2.44e-04 | −0.008 | −0.015, | 0.029 |
| −0.008 | −0.006 | −0.005 | −0.006 | −0.001 | |||||||||||
| cg13951797 (TRAF7) | −0.016 | −0.024, | 1.34e-05 | −0.011 | −0.018, | 0.003 | −0.008 | −0.016, | 0.033 | −0.007 | −0.015, | 0.065 | −0.009 | −0.016, | 0.025 |
| −0.009 | −0.004 | −0.001 | 4.46e-04 | −0.001 | |||||||||||
| cg03519879 (C14orf43) | −0.013 | −0.019, | 1.80e-05 | −0.014 | −0.021, | 6.36e-05 | −0.012 | −0.019, | 0.002 | −0.011 | −0.018, | 0.003 | −0.012 | −0.018, | 3.93e-04 |
| −0.007 | −0.007 | −0.004 | −0.004 | -0.005 | |||||||||||
| Current weekly smoking (n = 71 yes; n = 762 no) | |||||||||||||||
| Total sample size | 808 | ||||||||||||||
| cg05575921 (AHRR) | −0.040 | −0.051, | 3.10e-14 | −0.038 | −0.048, | 5.17e-13 | −0.036 | −0.047, | 9.39e-10 | −0.035 | −0.046, | 7.56e-10 | −0.033 | −0.046, | 7.35e-09 |
| −0.030 | −0.027 | −0.024 | −0.024 | −0.022 | |||||||||||
| Ever smoked weekly (n = 132 ever; n = 848 never) | |||||||||||||||
| Total sample size | 949 | ||||||||||||||
| Cg05575921 (AHRR) | −0.035 | −0.043, | 5.90e-17 | −0.032 | −0.024, | 8.88e-15 | −0.028 | −0.037, | 1.38e-09 | −0.028 | −0.037, | 4.05e-10 | −0.028 | −0.037, | 4.85e-10 |
| −0.027 | −0.040 | −0.019 | −0.019 | −0.019 | |||||||||||
| CG08331398 (PSMB8) | −0.020 | −0.028, | 4.88e-06 | −0.019 | −0.010, | 2.17e-05 | −0.022 | −0.031, | 1.36e-05 | −0.021 | −0.031, | 1.25e-05 | −0.020 | −0.030, | 3.84e-05 |
| −0.011 | −0.027 | −0.012 | −0.012 | −0.011 | |||||||||||
| Cotinine in the blood 10 ng/ml | |||||||||||||||
| Total sample size | 770 | ||||||||||||||
| CG05575921 (AHRR) | −0.004 | −0.005, | 4.19e-16 | −0.004 | −0.005, | 3.24e-13 | −0.003 | −0.004, | 6.01e-10 | −0.003 | −0.004, | 2.79e-10 | −0.003 | −0.004, | 1.41e-10 |
| −0.003 | −0.003 | −0.002 | −0.002 | −0.002 | |||||||||||
| CG06338710 (GFI1) | −0.006 | −0.008., | 1.73e-06 | −0.006 | −0.008, | 2.12e-06 | −0.006 | −0.008, | 2.91e-05 | −0.004 | −0.007 | 3.67e-04 | −0.004 | −0.006, | 7.30e-04 |
| −0.003 | −0.003 | −0.003 | −0.002 | −0.001 | |||||||||||
| CG09935388 (GFI1) | −0.007 | −0.009, | 2.98e-08 | −0.006 | −0.009, | 2.35e-07 | −0.006 | −0.009, | 1.15e-06 | −0.004 | −0.005, | 5.67e-05 | −0.005 | −0.007, | 4.86e-07 |
| −0.004 | −0.004 | −0.004 | −0.002 | −0.003 | |||||||||||
| CG12876356 (GFI1) | −0.007 | −0.010, | 1.74e-06 | −0.007 | −0.010, | 4.09e-06 | −0.007 | −0.010, | 2.01e-05 | −0.004 | −0.006, | 4.29e-04 | −0.005 | −0.007, | 2.81e-04 |
| −0.004 | −0.004 | −0.004 | −0.002 | −0.002 | |||||||||||
| CG18146737 (GFI1) | −0.006 | −0.008, | 1.29e-05 | −0.006 | −0.009, | 2.21e-05 | −0.006 | −0.009, | 4.19e-05 | −0.002 | −0.003, | 0.002 | −0.004 | −0.006, | 9.54e-04 |
| −0.003 | −0.003 | −0.003 | −7.47e-04 | −0.001 | |||||||||||
| CG26703534 (AHRR) | −0.003 | −0.004, | 3.11e-07 | −0.003 | −0.004, | 2.04e-06 | −0.002 | −0.004, | 1.17e-05 | −0.002 | −0.003, | 1.19e-05 | −0.002 | −0.004, | 1.26e-05 |
| −0.002 | −0.001 | −0.001 | −7.47e-04 | −0.001 | |||||||||||
Basic model: Adjusted model for batch and cell count. Adjusted model 1: Adjusted model for batch, cell count, age, sex, social class and maternal smoking during pregnancy. Adjusted model 2: Adjusted model for batch, cell count, age, sex, social class, regular cannabis use, regular alcohol use, passive smoke and maternal smoking during pregnancy. Adjusted model 3: Adjusted model for batch, cell count, age, sex, social class, regular cannabis use, regular alcohol use, passive smoke, maternal smoking during pregnancy and methylation at age 7. Adjusted model 4: Adjusted model for batch, cell count, age, sex, social class, regular cannabis use, regular alcohol use, passive smoke, maternal smoking during pregnancy and cord blood methylation.
Bonferroni threshold = 1.91e-05.