Figure 1.

TAZ and RunX2 activities are stimulated by the PC1-CTT. (A) HEK293 cells were transfected with TAZ-Gal4, UAS-Luciferase and Renilla luciferase reporter constructs alone or in the presence of the PC1-CTT or PC1-CTT∆NLS and luciferase activity was measured 24 h later. (B) Pkd1^flox/- and Pkd1^-/- cells stably expressing HA-PC1-CTT in a TET-Off inducible vector were transfected with RunX2-luciferase and Renilla reporter constructs in the presence or absence of doxycyclin to induce expression of the PC1-CTT and luciferase activity was measured 24 h later. (C) C3H10T^1/2 cells were reverse transfected (2× serial transfections) with either siControl (non-targeting RNA) or siRNA directed against mouse Pkd1. mRNA knockdown efficiency was assessed by qRT-PCR after 72 hrs. (D) C3H10T^1/2 cells were first reverse-transfected with either siControl or siPkd1 RNAi, then super-transfected with RunX2-luciferase and Renilla-luciferase alone or in the presence of the PC1-CTT. Luciferase values were measured 24 h after RunX2-lucferase transfection (72 h total after initial siRNA treatment). Results are expressed as mean ± standard error (SE) from nine biological replicates of three independent experiments.