Figure 2.

TAZ is required for PC1-CTT mediated stimulation of RunX2. (A) C3H10T1/2 cells were transfected with RunX2-luciferase, PC1-CTT, PC1-CTT∆NLS (negative control) and TAZ; luciferase activity was measured after 24 h. FLAG-RunX2 was over-expressed as a positive control for the RunX2-luciferase reporter (right panel). (B) C3H10T1/2 cells were reverse-transfected (2x serial transfections) with either siControl (non-targeting RNA) or siRNA directed against mouse TAZ. mRNA knockdown efficiency was assessed by qRT-PCR after 72 h. (C) C3H10T1/2 cells were first reverse-transfected with either siControl or siTAZ RNAi, then super-transfected with RunX2-luciferase and Renilla alone or in the presence of the PC1-CTT. Luciferase values were measured 24 hrs after RunX2-lucferase transfection (72 h total after initial siRNA treatment). Results are expressed as mean ± SE from nine biological replicates of 3 independent experiments.