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. 2018 Dec 14;10:6971–6984. doi: 10.2147/CMAR.S182852

Figure 2.

Figure 2

Knockdown of CENPU by RNA interference inhibits proliferation and induces G1 arrest in lung cancer cells.

Notes: (A) Quantitative reverse transcription PCR results showed that the mRNA expression level of CENPU was decreased in the siCENPU group compared with that in the NC group in SPC-A1 and A549 lung cancer cells 48 hours after transfection. (B) Western blotting was performed to analyze the expression level of CENPU 48 hours after siRNA transfection. (C) CCK-8 assays were performed to evaluate the effect of CENPU knockdown on the growth of SPC-A1 and A549 cells. (D, E) Cell proliferation rates were determined by EdU assays in SPC-A1 and A549 cells between the siCENPU and NC groups. (E) Cell cycle analysis of SPC-A1 and A549 cells transfected with siCENPU or NC siRNA. ****P<0.0001; ***P<0.001; **P<0.01 and *P<0.05 (mean ± SEM). (F) Quantitative reverse transcription results showed that the mRNA expression level of CENPU was increased in the CENPU group compared with that in the NC group in SPC-A1 cells 48 hours after transfection. (G) Western blotting showed that the expression of CENPU protein increased 48 hours after CENPU plasmid transfection in SPC-A1 cells. (H) Cell cycle analysis of SPC-A1 cells transfected with CENPU plasmid or NC plasmid.

Abbreviations: CCK-8, Cell Counting Kit-8; CENPU, centromere protein U; EdU, 5-ethynyl-2’-deoxyuridine; NC, negative control; SEM, standard error of the mean; siCENPU, CENPU siRNA.