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. 2018 Dec 14;10:6971–6984. doi: 10.2147/CMAR.S182852

Figure 4.

Figure 4

Knockdown of CENPU inhibits FOXM1 expression in lung cancer cells.

Notes: (A) Quantitative reverse transcription results showed that the mRNA expression of CENPU was downregulated in the siCENPU group compared with that expression in the NC group in SPC-A1 and A549 lung cancer cells. (B) Western blotting showed that the expression of CENPU declined 48 hours after siCENPU transfection in SPC-A1 and A549 cells. (C) Western blotting showed that the protein expression of FOXM1 was significantly downregulated in the siCENPU group. (D) Relative protein levels of FOXM1 were determined by Western blotting. The protein levels of FOXM1 were normalized to those of β-actin. (E) Quantitative reverse transcription PCR results showed that the mRNA expression of FOXM1 was downregulated in the siFOXM1 group compared with that expression in the NC group. (F) Western blotting showed that the expression of FOXM1 protein declined 48 hours after siFOXM1 transfection. (G) Knockdown of FOXM1 did not affect the mRNA expression level of CENPU, which was determined by RT-qPCR. (H) Western blotting showed that the expression of CENPU protein was not changed in the siFOXM1 group in both SPC-A1 and A549 cells; ***P<0.001; **P<0.01; and *P<0.05 (mean ± SEM).

Abbreviations: CENPU, centromere protein U; FOXM1, Forkhead box protein M1; NC, negative control; NS, no significance; RT-qPCR, real-time quantitative PCR; SEM, standard error of the mean; siCENPU, CENPU siRNA; siFOXM1, FOXM1 siRNA.